other:Article Title: The Molecular Chaperone TCP1 Affects Carcinogenicity and Is a Potential Therapeutic Target for Acute Myeloid Leukemia
Article Snippet: The following antibodies were used: anti-TCP1 polyclonal antibody (Proteintech, 10320-1-AP); anti-cleaved-caspase-3 (Wanleibio, WL02117); anti-CDC20 Rabbit pAb (Abclonal, A15656); anti-PKMYT1 Rabbit pAb (Abclonal, A20525); anti-GAPDH monoclonal antibody (ImmunoWay, YM3029); anti-AML1-ETO (CST, 4334); anti-cleaved-caspase-9 (CST, 52873); anti-cleaved-PARP (CST, 5625); anti-PLK1 (Abcam, ab189139); anti-pan-myc (Abcam, Ab195207 ); anti-mouse IgG, HRP-conjugated (CST, 7076); and anti-rabbit IgG, HRP-conjugated (CST, 7074).
Article Title: Linking kinetochore attachment to checkpoint control: the role of Aurora B in BubR1 acetylation
Article Snippet: Commercially sourced antibodies used in this study include anti-BubR1 (612 503; BD Biosciences), anti-α-tubulin-FITC (F2168; Upstate), anti-Centromere protein antibody (15–235; Antibodies Inc.), anti-HDAC2 (ab12169; Abcam), anti-HDAC3 (ab7030; Abcam), anti-CDC20 (A15656; ABclonal), anti-MAD2 (A11469; ABclonal), anti-Cyclin B (sc-245; Santa Cruz Biotechnology), anti-β-actin (A700-057; Bethyl), anti-CDC27 (BD 610455; BD Biosciences), anti-FLAG (F1804; Sigma–Aldrich), anti-GAPDH (#2118; Cell Signaling Technology), anti-CENP-A (ab13939;Abcam), anti-ZW10 (sc-81430; Santa Cruz Biotechnology), anti-Myc (sc-40; Santa Cruz Biotechnology), anti-AIM-1 (BD611082; BD Biosciences), anti-Vinculin (sc-73614; Santa Cruz Biotechnology), anti-AurB-pT232 (636 102; BioLegend), anti-PLK1 (ab17057; Abcam), anti-mCherry (ab125096; Abcam), anti-Bub3 (611 730; BD Biosciences), ani-CENP-E (A15263; ABclonal), Cyclin B (sc-245; Santa Cruz Biotechnology), anti-pH3 (06–570; EMD Millipore), anti-pan-Phospho-serine (AP0932; ABclonal), and anti-Cdc25C (A12234, ABclonal).
Article Title: APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons.
Article Snippet: Input and eluted pulldown samples were run on 4–20% Criterion TGX gels (Bio-Rad) and transferred to nitrocellulose membranes (GE Healthcare) and western blots were performed with rabbit anti-APC2 (1:500, ABclonal, A8066), rabbit anti-APC5 (1:500, ABclonal, A7109), rabbit anti-CDC20 (1:500, ABclonal, A15656), or mouse anti-H3 (1:1,000, Cell Signaling Technologies, 3638S) primary antibodies and IRDye 800CW goat anti-rabbit IgG (1:10,000, LICOR, 926-32211) or goat anti-mouse IgG (1:10,000, LI-COR, 926-32210) secondary antibodies.
Article Title: Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding
Article Snippet: Primary antibodies included: rabbit anti-RNF2 (1:500 dilution, ABclonal, catalog no. A5563), rabbit anti-VRK1 (1:500, ABclonal, A7745), rabbit anti-Ran (1:1000, ABclonal, A0976), rabbit anti-DMAP1 (1:500, ABclonal, A2324), rabbit anti-BAZ1B (1:125, ABclonal, A9851), rabbit anti-KIF20B (1:500, ABclonal, A15360), rabbit anti-TADA2A (1:150, ABclonal, A8457), rabbit anti-TADA3 (1:500, ABclonal, A6224), rabbit anti-RUVBL2 (1:350, ABclonal, A1905), rabbit anti-PARP1 (1:500, ABclonal, A11010), rabbit anti-NUP160 (1:375, ABclonal, A13080), rabbit anti-CDC20 (1:600, ABclonal, A15656), rabbit anti-BARD1 (1:1000, ABclonal, A1685), rabbit anti-NUP107 (1:500, ABclonal, A13110), rabbit anti-NUP85 (1:500, ABclonal, A11629), rabbit anti-AHCTF1 (1:150, Sigma, HPA031658), mouse anti-CDC27 (1:375, Santa Cruz, sc-9972), rabbit anti-ACTR6 (1:1000, ABclonal, A13227), rabbit anti-CDC16 (1:500, ABclonal, A7197), rabbit anti-SEC13 (1:1000, ABclonal, A11613), rabbit anti-NUP133 (1:500, ABclonal, A8818), rabbit anti-ANAPC2 (1:500, ABclonal, A8066), rabbit anti-NUP160 (1:500, ABclonal, A130080), rabbit anti-RAD50 (1:250, ABclonal, A3078), rabbit anti-RIF1 (1:100, GeneTex, GTX48737), rabbit anti-SMARCC1 (1:500, Santa Cruz, SC-10756), mouse anti-RCC1 (1:1000, Sigma, SAB1403666), rabbit anti-ANAPC5 (1:500, ABclonal, A7109), rabbit anti-SMC1A (1:2000, Bethyl, A300-055A), rabbit anti-TRIP12 (1:500, Aviva, OAAB20005), mouse anti-TET1 (1:100, GeneTex, GTX627420), mouse anti-OGT (1:250, GeneTex, GTX629813), rabbit anti-WDR76 (1:300, Sigma, HPA039804), rabbit anti-DNMT1 (1:300, Sigma, D4692), rabbit anti-MPHOSPH8 (1:500, Proteintech, 16796-1-AP), rabbit anti-Histone H3 (1:1000, Abcam, ab18521) and mouse anti-BUBR1 (1:500, gift from McKeon Lab) ( ).
Incubation:Article Title: STEAP4 with copper reductase activity suppresses tumorigenesis by regulating the cell cycle in hepatocellular carcinoma cells.
Article Snippet: .. The tissue section was then incubated overnight at 4 °C with an anti-STEAP4 antibody (A17767, 1:100, Abclonal, Germany), anti-CDC20 antibody (A15656, 1:100, Abclonal, Germany), anti-CDK1 antibody (A22347, 1:100, Abclonal, Germany), anti-Cyclin B2 antibody (DF9047, 1:100, Affinity Biosciences, USA), anti-PLK1 antibody (AF2685, 1:100, Affinity Biosciences, USA), and anti-CDC25C antibody (AF6258, 1:100, Affinity Biosciences, USA). ..
Western Blot:Article Title: APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons
Article Snippet: .. Input and eluted pulldown samples were run on 4–20% Criterion TGX gels (Bio-Rad) and transferred to nitrocellulose membranes (GE Healthcare) and western blots were performed with rabbit anti-APC2 (1:500, ABclonal, A8066), rabbit anti-APC5 (1:500, ABclonal, A7109), rabbit anti-CDC20 (1:500, ABclonal, A15656), or mouse anti-H3 (1:1,000, Cell Signaling Technologies, 3638S) primary antibodies and IRDye 800CW goat anti-rabbit IgG (1:10,000, LI-COR, 926-32211) or goat anti-mouse IgG (1:10,000, LI-COR, 926-32210) secondary antibodies. .. Western blots were imaged using an Odyssey imager (LI-COR).
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